<em>In vivo</em> Optogenetic Stimulation of the Rodent Central Nervous System

Michelle M. Sidor; Thomas J. Davidson; Kay M. Tye; Melissa R. Warden; Karl Diesseroth; Colleen A. McClung

doi:10.3791/51483

Journal

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神经科学

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在啮齿动物中枢神经系统的体内光遗传学刺激

<em>In vivo</em> Optogenetic Stimulation of the Rodent Central Nervous System

JoVE 杂志
神经科学

This content is Free Access.

JoVE 杂志神经科学

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

在啮齿动物中枢神经系统的体内光遗传学刺激

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59,093 Views

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09:37 min

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January 15, 2015

DOI:

10.3791/51483-v

DOI:

10.3791/51483-v

•

09:37 min

January 15, 2015

•

17 Views

Michelle M. Sidor¹, Thomas J. Davidson², Kay M. Tye³, Melissa R. Warden⁴, Karl Diesseroth^2,5, Colleen A. McClung¹

¹Department of Psychiatry,University of Pittsburgh Medical Center, ²Department of Bioengineering,Stanford University, ³Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory,Massachusetts Institute of Technology, ⁴Department of Neurobiology and Behavior,Cornell University, ⁵Department of Psychiatry and Behavioral Sciences,Stanford University

Summary

Optogenetics has become a powerful tool for use in behavioral neuroscience experiments. This protocol offers a step-by-step guide to the design and set-up of laser systems, and provides a full protocol for carrying out multiple and simultaneous in vivo optogenetic stimulations compatible with most rodent behavioral testing paradigms.