Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Kevin G. Chen; Rebecca S. Hamilton; Pamela G. Robey; Barbara S. Mallon

doi:10.3791/51519

JoVE Journal
Biology

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JoVE Journal Biology

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

另类文化的人多能干细胞的生产，维护和遗传分析

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08:27 min

July 24, 2014

DOI: 10.3791/51519-v

Kevin G. Chen¹, Rebecca S. Hamilton¹, Pamela G. Robey², Barbara S. Mallon¹

¹NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke,National Institutes of Health, ²Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research,National Institutes of Health

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在这里，我们提出了基于解离单细胞的非集落型单层（NCM）生长的人多能干细胞（hPSC）培养方案。这种利用 Rho 相关激酶抑制剂或层粘连蛋白亚型 521 （LN-521）的新方法适用于产生大量均相 hPSC、基因作和药物发现。

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