Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

Shannon N. Romano; Daniel A. Gorelick

doi:10.3791/51533

JoVE Journal
Biology

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Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

组织特异性荧光的斑马鱼胚胎半自动成像

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07:06 min

May 17, 2014

DOI: 10.3791/51533-v

Shannon N. Romano¹, Daniel A. Gorelick¹

¹Department of Pharmacology and Toxicology,University of Alabama at Birmingham

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这里描述的是一种协议，组织特异性荧光的斑马鱼胚胎半自动化成像。

该程序的总体目标是自动检测活斑马鱼幼虫中的雌激素反应细胞。这是通过首先培育五条 X-E-R-E-G-F-P 成鱼来产生雌激素报告斑马鱼胚胎来实现的。接下来，将胚胎置于 96 孔板的各个孔中。

然后将雌激素添加到胚胎板中。最后，使用带有电动载物台的落射荧光显微镜扫描板的荧光。最终，荧光显微镜用于显示雌激素受体的组织特异性活性。

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