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Bioengineering
保利的静电纤维支架(甘油 - 十二双)工程神经组织从小鼠胚胎干细胞
保利的静电纤维支架(甘油 - 十二双)工程神经组织从小鼠胚胎干细胞
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells

保利的静电纤维支架(甘油 - 十二双)工程神经组织从小鼠胚胎干细胞

Full Text
11,360 Views
08:03 min
June 18, 2014

DOI: 10.3791/51587-v

Xizi Dai1, Yen-Chih Huang1

1Department of Biomedical Engineering,Florida International University

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Please note that some of the translations on this page are AI generated. Click here for the English version.

静电合成长纤维经过了全新设计的收集器从名为聚(甘油 - 十二烷酸)一种新型的可生物降解聚合物跨越一个较大的矿床区(PGD),并制作报告。该纤维为能够支持从小鼠多能干细胞衍生的细胞的生长。

该程序使用一种名为聚甘油 Dote Cano eight 的新型可生物降解聚合物制造 Electros 纺长纤维。首先设置新设计的收集器并准备用于静电纺丝的聚合物溶液,使用注射器和泵将溶液输送到收集器。然后将所得纤维垫转移到培养皿中进行交联。

切割的纤维上接种小鼠胚胎干细胞。最终,结果可以证明小鼠胚胎干细胞可以存活并在纤维支架上分化为神经细胞。在今天的视频中,我们向您展示了如何从 syn 细胞制造中制备生物材料,最后使用这些电跨度纤维进行细胞培养。

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