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Chapters
- 00:04Title
- 01:38Dissection of Thyroid Tissue
- 04:58Plating and Culture of Thyroid Explants
- 06:47Results: Thyroid Folliculogenesis, Angiogenesis, and Differentiation in Culture
- 08:04Conclusion
This protocol describes dissection of mouse embryonic thyroid anlagen and the culture of explants on semiporous filters or on microscopy plastic slides. This system is ideal to study morphogenetic or differentiation events occurring during thyroid development of wild type or knockout embryos, and is amenable to gain- and loss-of-function experiments.
