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Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration
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生物学
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JoVE 杂志 生物学
Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

DOI:
10.3791/51682-v

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10:35 min

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September 06, 2014

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, , , , ,
1Division of Cardiothoracic Surgery,Beth Israel Deaconess Medical Center and Harvard Medical School, 2Department of Anesthesiology, Perioperative and Pain Medicine,Boston Children’s Hospital and Department of Anesthesia, Harvard Medical School

Chapters

  • 00:05Title
  • 01:21Harvesting Mitochondria
  • 04:32The ATP Assay
  • 08:18Mitochondria Characterization from Liver and Skeletal Muscle Biopsy
  • 10:16Conclusion

Summary

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A method for rapid isolation of mitochondria from mammalian tissue biopsies is described. Rat liver or skeletal muscle preparations were homogenized with a commercial tissue dissociator and mitochondria were isolated by differential filtration through nylon mesh filters. Mitochondrial isolation time is <30 min compared to 60 - 100 min using alternative methods.

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Mitochondrial IsolationRapid IsolationDifferential FiltrationBiopsyNylon Mesh FiltersRespiration Competent Mitochondria

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