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造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪
<em>In Vivo</em> 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

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13,789 Views

12:54 min

September 04, 2014

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12:54 min
September 04, 2014

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Summary

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造血干细胞和祖细胞(HSPCs)和骨髓龛之间的相互作用的性质知之甚少。定制的硬件修改和多步获取协议允许使用双光子共焦显微术图像体外骨髓区域内驻留标记HSPCs,跟踪相互作用和运动。

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