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DOI: 10.3791/51738-v
Marc Cazaux*1,2, Marie Navarro*1,2, Kristie A. Bruinsma*1, Vladimir Zhurov*1, Tara Negrave1, Thomas Van Leeuwen3,4, Vojislava Grbic1,2, Miodrag Grbic1,2
1Department of Biology,The University of Western Ontario, 2Instituto de Ciencias de la Vid y el Vino, 3Department of Crop Protection,Ghent University, 4Institute for Biodiversity and Ecosystem Dynamics,University of Amsterdam
Please note that some of the translations on this page are AI generated. Click here for the English version.
协议对红蜘蛛,实验植物的侵染和植物的损害评估的均匀样品的制备效率的要求,对植物病虫害的相互作用研究,开发。
该程序的总体目标是在给定的发育阶段快速可靠地收集蜘蛛螨。这是通过首先在豆类植物上佩戴螨虫来实现的。接下来,使用画笔或泵在实验植物中感染雌性螨虫,具体取决于要应用于单个植物的螨虫数量。
最后一步是在图形编辑程序中使用网格方法手动量化植物损伤。最终,统计分析用于显示植物品系或生态型对蜘蛛螨的差异敏感性。与现有方法相比,该技术的优势在于,它提供了一种有效的方法,可以快速轻松地收集大量成年雌螨,将它们应用于实验植物宿主,并评估由于螨虫喂养而导致的植物损害。
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