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二斑叶螨的应用二斑叶螨植物病虫害的相互作用研究
二斑叶螨的应用二斑叶螨植物病虫害的相互作用研究
JoVE Journal
Environment
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JoVE Journal Environment
Application of Two-spotted Spider Mite Tetranychus urticae for Plant-pest Interaction Studies

二斑叶螨的应用二斑叶螨植物病虫害的相互作用研究

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18,304 Views
08:53 min
July 4, 2014

DOI: 10.3791/51738-v

Marc Cazaux*1,2, Marie Navarro*1,2, Kristie A. Bruinsma*1, Vladimir Zhurov*1, Tara Negrave1, Thomas Van Leeuwen3,4, Vojislava Grbic1,2, Miodrag Grbic1,2

1Department of Biology,The University of Western Ontario, 2Instituto de Ciencias de la Vid y el Vino, 3Department of Crop Protection,Ghent University, 4Institute for Biodiversity and Ecosystem Dynamics,University of Amsterdam

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Please note that some of the translations on this page are AI generated. Click here for the English version.

协议对红蜘蛛,实验植物的侵染和植物的损害评估的均匀样品的制备效率的要求,对植物病虫害的相互作用研究,开发。

该程序的总体目标是在给定的发育阶段快速可靠地收集蜘蛛螨。这是通过首先在豆类植物上佩戴螨虫来实现的。接下来,使用画笔或泵在实验植物中感染雌性螨虫,具体取决于要应用于单个植物的螨虫数量。

最后一步是在图形编辑程序中使用网格方法手动量化植物损伤。最终,统计分析用于显示植物品系或生态型对蜘蛛螨的差异敏感性。与现有方法相比,该技术的优势在于,它提供了一种有效的方法,可以快速轻松地收集大量成年雌螨,将它们应用于实验植物宿主,并评估由于螨虫喂养而导致的植物损害。

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环境科学 第89期 二斑叶螨 植物 - 草食动物互动 二斑叶螨拟南芥 对植物的损伤分析 虫食 植物病虫害

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