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Assessment of Murine Exercise Endurance Without the Use of a Shock Grid: An Alternative to Forced Exercise

Article DOI: 10.3791/51846-v • 07:52 min • August 14th, 2014
August 14th, 2014 •
1,2, 1, 1,3,4
1Research Service, VA Puget Sound Health Care System, 2Seattle Institute for Biomedical and Clinical Research, 3Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Washington, 4Geriatric Research, Education, and Clinical Center, VA Puget Sound Health Care System

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A method to assess exercise endurance in laboratory mice without the use of a shock grid is demonstrated. This method is a humane refinement that can decrease the confounding effects of stress on experimental parameters.

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Exercise Endurance Murine Models Metabolic Benefits Molecular Pathways Forced Exercise Shock Grid Alternative Method Treadmill Running Electric Foot Shock Avoidance Behavior Psychological Stress Quantitation Serum Hormone Levels Cytokine Levels Refined Method Voluntary Running Gentle Encouragement Human Operator Tongue Depressor Willingness To Run Running Time-to-exhaustion Behavioral Signs Of Exhaustion
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