Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Xiaobei Zhao; Yue Zhou; April M. Weissmiller; Matthew L. Pearn; William C. Mobley; Chengbiao Wu

doi:10.3791/51899

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

初级量子神经元轴突运输的实时成像点标记的BDNF

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10:53 min

September 15, 2014

DOI: 10.3791/51899-v

Xiaobei Zhao¹, Yue Zhou², April M. Weissmiller¹, Matthew L. Pearn^3,4, William C. Mobley¹, Chengbiao Wu¹

¹Department of Neurosciences,University of California, San Diego, ²School of Biomedical Engineering and Med-X Research Institute,Shanghai Jiao Tong University, ³Department of Anesthesiology,University of California, San Diego, ⁴VA San Diego Healthcare System

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BDNF，神经营养因子，对轴突运输是几个神经元群体的生存和功能是至关重要的。一些退行性疾病的标志是轴索结构和功能的破坏。我们展示了用于检查QD-BDNF在使用初级神经元微流控室现场贩卖的技术。

该程序的总体目标是通过实时成像跟踪脑源性神经营养因子或 BDNF 的逆行轴突转运。这是通过首先在聚赖氨酸涂层的盖玻片上组装微流体室来实现的。在第二步中，解剖解离的大鼠 E 18 海马神经元并接种在微流体室的细胞体隔室中。

接下来，对神经元进行培养，以允许轴突穿过微沟延伸到腔室的轴突隔室。在最后一步中，将标记的 BDNF 量子点添加到轴突室中。最终，荧光显微镜可用于评估 BDNF 在海马神经元轴突内的运动。

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神经科学第91 实时成像脑源性神经营养因子（BDNF）量子点贩卖轴突逆行运输微流控室