JoVE Journal
Developmental Biology
Developmental Biology
需要订阅 JoVE 才能查看此 内容.
章节
总结概括
Embryonic chick retinal cell cultures constitute valuable tools for the study of photoreceptor biology. We have developed an efficient gene transfer technique based on ex ovo plasmid electroporation of the retinas prior to culture. This technique considerably increases transfection efficiencies over currently available protocols, making genetic manipulation feasible for this system.