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The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
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The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

Article DOI: 10.3791/52113-v • 10:26 min • December 19th, 2014
December 19th, 2014 •
1, 2, 1, 3, 1
1Department of Plant Sciences, University of Oxford, 2School of Education of Vitoria-Gasteiz, University of the Basque Country (UPV/EHU), 3Biosciences, University of Exeter

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This protocol details the optimized extraction of apoplast washing fluid from plant leaves, using French bean plants (Phaseolus vulgaris) as a model example.

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Infiltration-centrifugation Technique Extraction Apoplastic Fluid Plant Leaves Phaseolus Vulgaris Apoplast Cell Wall Formation Nutrient And Water Uptake Plant-endophyte Interactions Defence Responses Pathogens Apoplast Washing Fluid (AWF) Vacuum Infiltration Centrifugation Method French Bean Tendergreen Leaves Downstream Experiments Composition Of The Apoplast Plant Species And Genotype Plant Development Environmental Conditions Microorganisms
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