基于社区环境采集样本的全血成分快速分离与提取
16.1K 次观看
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被引用 19 次
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11:31 分钟
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2015年11月30日
DOI :10.3791/52227-v
2015年11月30日
•16.1K 次观看
Amy Weckle1,2 , Allison E. Aiello3 , Monica Uddin1,4 , Sandro Galea5 , Rebecca M. Coulborn6 , Richelo Soliven7 , Helen Meier6 , Derek E. Wildman1,2
1Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 2Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, 3Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, 4Department of Psychology, University of Illinois at Urbana-Champaign, 5Department of Epidemiology, Mailman School of Public Health, Columbia University, 6Department of Epidemiology, University of Michigan School of Public Health, 7Center for Molecular Medicine and Genetics, Wayne State University School of Medicine
我们概述了一种用于处理全血以获取多种组分以供进一步分析的方法。我们优化了一个简化的操作流程，可在非临床环境下快速、高通量地同时处理多个全血样本。
Chapters in this video
0:05
Title
2:09
Serum, DNA, and PBMC Isolation Stage 1
3:49
DNA Isolation Stage 1
4:36
Leukocyte RNA Isolation Stage 1
6:02
PBMC Isolation Stage 2
8:06
Serum Isolation Stage 2 and PBMC Isolation Stage 3
10:30
Results: Ideal Leukocyte RNA Processing Yields
10:50
Conclusion
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