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Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain
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神经科学
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JoVE 杂志 神经科学
Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain

Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain

DOI:
10.3791/52271-v

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07:52 min

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July 07, 2015

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, , ,
1École d’optométrie,Université de Montréal

Chapters

  • 00:05Title
  • 01:50Preparing the Injectrode (Recording-injection Pipette)
  • 03:15Preparing the Animal
  • 04:51Performing the Experiment
  • 07:05Results: GABA Inhibition of Multi-unit Activity in the Superior Colliculus
  • 07:27Conclusion

Summary

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Here, we craft a glass pipette with dual functions: inhibition of deep brain structures by microinjections of drugs and real-time monitoring of their effects through simultaneous electrophysiological recordings.

Tags

Simultaneous Electrophysiological RecordingMicro-injectionsInhibitory AgentsRodent BrainInjectrodeMulti-unit Extracellular SignalsDrug DeliverySuperior ColliculusNeuronal Activity

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