Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

Maria Weinert; Tharakeswari Selvakumar; Travis S. Tierney; Kambiz N. Alavian

doi:10.3791/52475

Journal

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神经科学

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原发性脑多巴胺能神经元的分离，培养和长期维持胚胎脑鼠类

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

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神经科学

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JoVE 杂志神经科学

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

原发性脑多巴胺能神经元的分离，培养和长期维持胚胎脑鼠类

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13,993 Views

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08:45 min

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February 19, 2015

DOI:

10.3791/52475-v

DOI:

10.3791/52475-v

•

08:45 min

February 19, 2015

•

13932 Views

Maria Weinert¹, Tharakeswari Selvakumar², Travis S. Tierney², Kambiz N. Alavian^1,3

¹Division of Brain Sciences, Department of Medicine,Imperial College London ²Department of Neurosurgery,Brigham and Women’s Hospital, Harvard Medical School ³Department of Internal Medicine, Endocrinology,Yale University School of Medicine

Summary

The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of neurodegeneration.