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Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes
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JoVE 杂志 免疫与感染
Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes

Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes

DOI:
10.3791/52497-v

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09:18 min

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May 27, 2015

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,
1Department of Microbiology and Immunology,College of Medicine, University of Illinois at Chicago

Chapters

  • 00:05Title
  • 01:54Invasion Assay
  • 04:09Inoculating L. monocytogenes into Mice via Tail Vein Injection and Assessing Bacterial Burden in Liver, Spleen, and Heart
  • 07:39Results: L. monocytogenes Infection in Cultured Organ Cells
  • 08:47Conclusion

Summary

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Listeria monocytogenes causes fetal infections in pregnant women and meningitis in susceptible populations. Subpopulations of bacteria can colonize cardiac tissue, causing myocarditis in patients and laboratory animals. Here we present a protocol that describes how to assess L. monocytogenes cardiac cell invasion in vitro and cardiac colonization in infected animals.

Tags

Listeria MonocytogenesCardiac CellsHeart ColonizationCardiotropismH9c2 Cell LineMouse ModelIn VitroIn VivoInvasive InfectionListeriosisCardiac Pathology

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