Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Balakrishnan S. Moorthy; Lavanya K. Iyer; Elizabeth M. Topp

doi:10.3791/52503

Journal

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化学

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质谱途径研究蛋白质结构和相互作用的冻干粉

JoVE 杂志
化学

This content is Free Access.

JoVE 杂志化学

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

http://jovecomapi-svc-main-service/api/free/article/cn/52503
质谱途径研究蛋白质结构和相互作用的冻干粉

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15,570 Views

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11:14 min

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April 14, 2015

DOI:

10.3791/52503-v

DOI:

10.3791/52503-v

•

11:14 min

April 14, 2015

•

5 Views

Balakrishnan S. Moorthy*¹, Lavanya K. Iyer*¹, Elizabeth M. Topp¹

¹Department of Industrial and Physical Pharmacy,Purdue University

Summary

Here, we present detailed protocols for solid-state amide hydrogen/deuterium exchange mass spectrometry (ssHDX-MS) and solid-state photolytic labeling mass spectrometry (ssPL-MS) for proteins in solid powders. The methods provide high-resolution information on protein conformation and interactions in the amorphous solid-state, which may be useful in formulation design.