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Bioengineering
微流体芯片ICPMS进样
微流体芯片ICPMS进样
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Microfluidic Chip for ICPMS Sample Introduction

微流体芯片ICPMS进样

Full Text
11,818 Views
11:16 min
March 5, 2015

DOI: 10.3791/52525-v

Pascal E. Verboket1, Olga Borovinskaya1, Nicole Meyer1, Detlef Günther1, Petra S. Dittrich1

1Department of Chemistry and Applied Biosciences,ETH Zurich

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Please note that some of the translations on this page are AI generated. Click here for the English version.

我们提出了一个离散的微滴样品引入系统,用于电感耦合等离子体质谱法(ICPMS)。它是基于一种廉价和一次性的微流体芯片,其生成高度单分散的液滴中的40-60微米的尺寸范围的频率范围从90至7000赫兹上。

该程序的总体目标是通过 I-C-P-M-S 生成并定量分析含有单细胞或水溶液的单分散微滴的元素组成。这是通过首先使用 PDMS 的复制成型制造微流体芯片来实现的。第二步是将注射泵连接到微流控芯片。

泵将供应水性样品以及高挥发性和可接受或有机载体相 PFH。当注射泵打开时,微流体芯片通过流动产生水性样品溶液或细胞悬液的单分散液滴,用 PFH 聚焦,并以液体射流的形式从芯片中喷出这些液滴和额外的 PFH。最后一步是将芯片插入运输组件中,该组件由蒸发液滴的筒式加热器和在电离和通过 I-C-P-M-S 检测液滴含量之前去除溶剂的膜 de salvator 组成。

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