多器官芯片 - 一种微流体平台的长期多组织共培养

Overview

This article presents a protocol for coculturing primary cells, tissue models, and punch biopsies using a microfluidic multi-organ chip for up to 28 days. The successful combination of human dermal microvascular endothelial cells, liver aggregates, and skin biopsies in a common media circulation is detailed.

Key Study Components

Area of Science

• Neuroscience
• Cell Biology
• Microfluidics

Background

• Microfluidic systems allow for the integration of multiple organ systems.
• Coculture techniques can enhance the study of cellular interactions.
• Maintaining cell viability and functionality over extended periods is crucial.
• This study explores the potential of a multi-organ chip for long-term culture.

Purpose of Study

• To develop a reliable protocol for coculturing different cell types.
• To investigate the interactions between endothelial cells and tissue models.
• To assess the viability of cells in a microfluidic environment over time.

Methods Used

• Microfluidic chip design and fabrication.
• Isolation of primary human dermal microvascular endothelial cells.
• Preparation of liver aggregates and skin biopsies.
• Establishment of common media circulation for coculture.

Main Results

• Successful coculture of endothelial cells, liver aggregates, and skin biopsies.
• Cells maintained viability for up to 28 days.
• Observations of cellular interactions in the microfluidic environment.
• Potential applications in drug testing and disease modeling.

Conclusions

• The microfluidic multi-organ chip is effective for long-term coculture.
• This approach can facilitate the study of complex cellular interactions.
• Future research may expand on the applications of this technology.

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