Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens

Christopher P. Barkway; Rebecca L. Pocock; Vladimir Vrba; Damer P. Blake

doi:10.3791/52552

Journal

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免疫与感染

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环介导等温扩增（LAMP）法进行的物种特异性检测艾美耳球虫可感染鸡

JoVE 杂志
免疫与感染

This content is Free Access.

JoVE 杂志免疫与感染

Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens

环介导等温扩增（LAMP）法进行的物种特异性检测艾美耳球虫可感染鸡

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26,827 Views

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06:57 min

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February 20, 2015

DOI:

10.3791/52552-v

DOI:

10.3791/52552-v

•

06:57 min

February 20, 2015

•

14 Views

Christopher P. Barkway¹, Rebecca L. Pocock¹, Vladimir Vrba², Damer P. Blake¹

¹Department of Pathology and Pathogen Biology,Royal Veterinary College, London, ²BIOPHARM,Research Institute of Biopharmacy and Veterinary Drugs

Summary

Diagnosis of Eimeria infection in chickens remains demanding. Parasite morphology- and host pathology-led approaches are commonly inconclusive, while molecular approaches based on PCR have proven demanding in cost and expertise. The aim of this protocol is to establish loop-mediated isothermal amplification (LAMP) as a straightforward molecular diagnostic for eimerian infection.