An Assay for Measuring the Effects of Ethanol on the Locomotion Speed of <em>Caenorhabditis elegans</em>

Andrew G. Davies; GinaMari G. Blackwell; Richard C. Raabe; Jill C. Bettinger

doi:10.3791/52681

Journal

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神经科学

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测量乙醇的影响上的步态速度的测定<em>秀丽隐杆线虫</em

An Assay for Measuring the Effects of Ethanol on the Locomotion Speed of <em>Caenorhabditis elegans</em>

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神经科学

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JoVE 杂志神经科学

An Assay for Measuring the Effects of Ethanol on the Locomotion Speed of Caenorhabditis elegans

测量乙醇的影响上的步态速度的测定<em>秀丽隐杆线虫</em

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8,660 Views

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10:35 min

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April 09, 2015

DOI:

10.3791/52681-v

DOI:

10.3791/52681-v

•

10:35 min

April 09, 2015

•

8606 Views

Andrew G. Davies^1,2, GinaMari G. Blackwell^1,2, Richard C. Raabe¹, Jill C. Bettinger^1,2

¹Department of Pharmacology and Toxicology,Virginia Commonwealth University ²VCU Alcohol Research Center,Virginia Commonwealth University

Summary

C. elegans is a useful model for studying the effects of ethanol on behavior. We present a behavioral assay that quantifies the effects of ethanol on the locomotion speed of crawling worms; both initial sensitivity and the development of acute functional tolerance to ethanol can be measured with this assay.