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Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke
JoVE 杂志
医学
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JoVE 杂志 医学
Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

14,755 Views

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08:07 min

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June 23, 2015

DOI:

10.3791/52794-v

DOI:
10.3791/52794-v

•

08:07 min
June 23, 2015

•

10 Views
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1Department of Cellular and Structural Biology,The University of Texas Health Science Center San Antonio, 2School of Medicine,The University of Texas Health Science Center San Antonio, 3Cell & Tissue Imaging Center,St. Jude Children’s Research Hospital

Summary

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Here, we describe a semi-invasive optical microscopy approach for the induction of a Rose Bengal photothrombotic clot in the somatosensory cortex of a mouse in vivo. The technical aspects of the imaging procedure are described from induction of a photothrombotic event to application and data collection.

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Rose Bengal PhotothrombosisConfocal Optical ImagingIn VivoSingle Vessel StrokeCranial WindowSomatosensory CortexMouse ModelLongitudinal StudiesIschemic EventLaser ExcitationPhotosensitive DyeConfocal MicroscopyIn Vivo Imaging

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Cite this Article

Talley Watts, L., Zheng, W., Garling, R. J., Frohlich, V. C., Lechleiter, J. D. Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke. J. Vis. Exp. (100), e52794, doi:10.3791/52794 (2015).

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