19,119 Views
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08:52 min
August 16, 2015
DOI:
10.3791/52840-v
这个协议描述了使用溴脱氧尿苷(BrdU)标记的摄取,以允许细胞是在S期在时间上的特定点的时间的跟踪。此外DNA染料和抗体标记的方便了S期细胞的命运在稍后时间详细分析。
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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Cite this Article
Welschinger, R., Bendall, L. J. Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization. J. Vis. Exp. (102), e52840, doi:10.3791/52840 (2015).
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