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Behavior
简单和计算机辅助测试嗅觉的小鼠
简单和计算机辅助测试嗅觉的小鼠
JoVE Journal
Behavior
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JoVE Journal Behavior
Simple and Computer-assisted Olfactory Testing for Mice

简单和计算机辅助测试嗅觉的小鼠

Full Text
10,894 Views
06:40 min
June 15, 2015

DOI: 10.3791/52944-v

Emanuele Brai1, Lavinia Alberi1

1Unit of Anatomy, Department of Medicine,University of Fribourg

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Please note that some of the translations on this page are AI generated. Click here for the English version.

我们在小鼠中提出了一种简单且无偏见的嗅觉测试。通过该方案,可以在单次行为会话中评估嗅觉辨别、偏好、回避和对新气味的敏感性。该方法适用于单个实验者,分析基于计算机辅助视频处理。

以下实验的总体目标是测试小鼠的先天嗅觉辨别、嗅觉偏好或回避和嗅觉敏感性。这是通过将鼠标暴露在新型引诱剂或上诉剂和中性气味(即水)中来实现的。同时,摄像机记录了行为过程。

接下来,使用提供的宏在图像 J 中分析视频,以获得在气味和水参数中花费的时间。结果显示了基于事后计算机辅助分析的不同基因型与水相比对气味的偏好或回避行为。与使用专用竞技场和嗅觉相比,嗅觉测试的主要优点是它使用熟悉的竞技场,因此完成速度快,设置简单。

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