Instillation and Fixation Methods Useful in Mouse Lung Cancer Research

Nathachit Limjunyawong; Jason Mock; Wayne Mitzner

doi:10.3791/52964

JoVE Journal
Medicine

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JoVE Journal Medicine

Instillation and Fixation Methods Useful in Mouse Lung Cancer Research

灌输和固定方法有用的小鼠肺癌研究

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06:57 min

August 31, 2015

DOI: 10.3791/52964-v

Nathachit Limjunyawong¹, Jason Mock², Wayne Mitzner¹

¹Bloomberg School of Public Health, Environmental Health Sciences,Johns Hopkins University, ²Department of Medicine, Pulmonary Diseases and Critical Care Medicine,University of North Carolina School of Medicine

Please note that some of the translations on this page are AI generated. Click here for the English version.

本文的目的是描述简单的方法，将在设置和小鼠肺分析肺癌或其它病理极大地帮助。我们提出3个协议能够简单，可靠地进行肺滴注，固定，和肺容积的测量。

此处显示的程序的总体目标是演示简单的方法，这些方法将极大地帮助设置和分析患有肺癌或其他肺部疾病的小鼠。首先是一种直接将癌细胞或化学治疗注入肺部的方法。然后描述死后固定和肺体积测量。

这是通过首先用略微改良的静脉插管插管小鼠来实现的。接下来，通过套管将感兴趣的液体滴入肺部。然后，在所有体内实验完成后，老鼠就死了。

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