Journal
/
Bioengineering
/
Engineered 3D Silk-collagen-based Model of Polarized Neural Tissue
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Engineered 3D Silk-collagen-based Model of Polarized Neural Tissue

Engineered 3D Silk-collagen-based Model of Polarized Neural Tissue

DOI:
10.3791/52970-v

•

06:17 min

•

October 23, 2015

•

, , , , ,
1Department of Biomedical Engineering,Tufts University, 2Department of Pediatrics,University of Connecticut Health Center & Connecticut Children’s Medical Center

Chapters

  • 00:05Title
  • 01:16Preparation of Porous Scaffolds from Silk Solution
  • 02:50Seeding the Scaffold with Cells and Collagen Overlay
  • 04:45Results: A 3D Brain-like Tissue Model
  • 05:56Conclusion

Summary

Automatic Translation

Automatic Translation

Insight into the complex actions of the brain requires advanced research tools. Here we demonstrate a novel silk-collagen-based 3D engineered model of neural tissue resembling brain-like architecture. The model can be used to study neuronal network assembly, axonal guidance, cell-cell interactions and electrical activity.

Tags

Silk-collagenPolarized NeuronsBrain-derived NeuronsNeural NetworkAxonal GuidanceCell-cell InteractionCell-matrix InteractionElectrical Function

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/cn/52970?article_type=v

Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments
check_url/cn/52970?article_type=v

Protocol for Relative Hydrodynamic Assessment of Tri-leaflet Polymer Valves
check_url/cn/52970?article_type=v

Tissue Engineering of a Human 3D in vitro Tumor Test System
check_url/cn/52970?article_type=v

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
check_url/cn/52970?article_type=v

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model
check_url/cn/52970?article_type=v

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds
check_url/cn/52970?article_type=v

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization
check_url/cn/52970?article_type=v

Custom Engineered Tissue Culture Molds from Laser-etched Masters
check_url/cn/52970?article_type=v

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
check_url/cn/52970?article_type=v

Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats

Read Article