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DOI: 10.3791/53071-v
Please note that some of the translations on this page are AI generated. Click here for the English version.
中枢呼吸驱动位于脑干。通过在第四腹根上放置电极来记录来自离体脑干脊髓的自发呼吸运动输出。这种实验方法对于药理学研究或评估呼吸挑战和遗传作对节律性运动行为很有价值。
该程序的总体目标是记录在不同条件下没有外周 E 的情况下脑干呼吸核产生的节律。这是通过首先去除大鼠脑干所在的颈部和上背部来实现的。第二步是隔离神经系统,去除颅骨和椎骨。
接下来,将电极放在第四腹根并开始记录。最终,在离体脑干和脊髓上进行电生理记录。制剂用于显示脑干对各种刺激产生的节律。
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