Sandwich-like Microenvironments to Harness Cell/Material Interactions

Jos&#233; Ballester-Beltr&#225;n; Myriam Lebourg; Manuel Salmer&#243;n-S&#225;nchez

doi:10.3791/53090

Journal

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生物工程

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三明治般的微环境来驾驭细胞/材料相互作用

Sandwich-like Microenvironments to Harness Cell/Material Interactions

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生物工程

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JoVE 杂志生物工程

Sandwich-like Microenvironments to Harness Cell/Material Interactions

三明治般的微环境来驾驭细胞/材料相互作用

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7,539 Views

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06:50 min

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August 04, 2015

DOI:

10.3791/53090-v

DOI:

10.3791/53090-v

•

06:50 min

August 04, 2015

•

2 Views

José Ballester-Beltrán^1,2, Myriam Lebourg^1,3, Manuel Salmerón-Sánchez²

¹Center for Biomaterials and Tissue Engineering,Universitat Politècnica de València, ²Division of Biomedical Engineering, School of Engineering,University of Glasgow, ³Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)

Summary

The following protocol describes the procedure to assemble sandwich-like cultures to be used as an intermediate stage between bi-dimensional (2D) and three-dimensional (3D) cellular environments. The engineered systems can have applications in microscopy, biomechanics, biochemistry and cell biology assays.