Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Laura M. Ketelboeter; Sonia L. Bardy

doi:10.3791/53105

Journal

/

免疫与感染

/

方法来抑制细菌Pyomelanin生产和确定敏感性氧化应激的影响相应增加

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

JoVE 杂志
免疫与感染

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志免疫与感染

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

方法来抑制细菌Pyomelanin生产和确定敏感性氧化应激的影响相应增加

Please note that all translations are automatically generated. Click here for the English version.

8,141 Views

•

11:00 min

•

August 31, 2015

DOI:

10.3791/53105-v

DOI:

10.3791/53105-v

•

11:00 min

August 31, 2015

•

2 Views

Laura M. Ketelboeter¹, Sonia L. Bardy¹

¹Department of Biological Sciences,University of Wisconsin Milwaukee

Summary

Bacterial pyomelanin production results in increased resistance to oxidative stress and virulence. We report on techniques that can be used to determine inhibition of pyomelanin production and assay the resulting increase in sensitivity to oxidative stress in bacteria, as well as determine antibiotic minimum inhibitory concentration (MIC).