Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Sarah J. Smith; Eric J. Horstick; Ann E. Davidson; James Dowling

doi:10.3791/53183

Journal

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发育生物学

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斑马鱼幼虫骨骼肌诚信与伊文思蓝分析

JoVE 杂志
发育生物学

This content is Free Access.

JoVE 杂志发育生物学

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

斑马鱼幼虫骨骼肌诚信与伊文思蓝分析

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9,021 Views

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07:34 min

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November 30, 2015

DOI:

10.3791/53183-v

DOI:

10.3791/53183-v

•

07:34 min

November 30, 2015

•

6 Views

Sarah J. Smith*^1,2, Eric J. Horstick*^3,4, Ann E. Davidson^1,2, James Dowling^1,2,4

¹Program in Genetics & Genome Biology,The Hospital for Sick Children, ²Department of Molecular Genetics,The University of Toronto, ³Program in Genomics of Differentiation,Eunice Kennedy Shriver National Institute of Child Health and Human Development, ⁴Departments of Pediatrics and Neurology,University of Michigan

Summary

In this study, we describe a straightforward method to perform Evans Blue Dye (EBD) analysis on zebrafish larvae. This technique is a powerful tool for the characterization of skeletal muscle integrity and delineation of zebrafish models of muscular dystrophy, and is a valuable method for the development of novel therapeutics.