Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

Nabil E. Boutagy; Emily Pyne; George W. Rogers; Mostafa Ali; Matthew W. Hulver; Madlyn I. Frisard

doi:10.3791/53217

Journal

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生物学

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从小鼠骨骼肌高通量微孔板呼吸测量最低数量的隔离线粒体

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JoVE 杂志生物学

Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

从小鼠骨骼肌高通量微孔板呼吸测量最低数量的隔离线粒体

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12,702 Views

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10:12 min

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November 13, 2015

DOI:

10.3791/53217-v

DOI:

10.3791/53217-v

•

10:12 min

November 13, 2015

•

3 Views

Nabil E. Boutagy^1,2, Emily Pyne¹, George W. Rogers³, Mostafa Ali¹, Matthew W. Hulver^1,2, Madlyn I. Frisard^1,2

¹The Department of Human Nutrition, Foods, and Exercise,Virginia Tech, ²The Metabolic Phenotyping Core,Virginia Tech, ³Seahorse Bioscience

Summary

Here, we present a modification of a previously reported method that allows for the isolation of high quality and purified mitochondria from smaller quantities of mouse skeletal muscle. This procedure results in highly coupled mitochondria that respire with high function during microplate based respirometirc assays.