<em>In Vitro</em> Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling

Winston Elliott; Devon Scott-Drechsel; Wei Tan

doi:10.3791/53224

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling

在生理体外模型和病理血流应用的血管细胞重塑调查

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07:30 min

November 3, 2015

DOI: 10.3791/53224-v

Winston Elliott¹, Devon Scott-Drechsel², Wei Tan¹

¹Department of Mechanical Engineering,University of Colorado at Boulder, ²Product Development,Covidien, Ltd.

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该协议将复制的生理或病理血流体外在确定疾病的病理细胞应答提供帮助。通过引入压力衰减室血液泵的下游，穿过脉管的血流，可以概括和强加于血管内皮或模拟物共培养的单层。

以下实验的总体目标是观察不同脉动血流病理对内皮细胞的影响。体外研究。这是通过首先对载玻片进行化学处理并用蛋白质包被以确保细胞在流式细胞暴露过程中的附着来实现的。

作为第二步，确定流速的脉动指数，这允许将体外流速与文献中描述的值进行比较。接下来，将位于细胞的载玻片连接到流式系统，以测量细胞对流式病理的反应。结果显示，使用光学显微镜和免疫荧光染色，基于流速曲线的形态和蛋白质表达发生变化。

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