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JoVE Journal Bioengineering
A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
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A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Article DOI: 10.3791/53261-v • 08:34 min • October 16th, 2015
October 16th, 2015 •
1, 1, 2, 1, 1
1Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2School of Chemical & Biomedical Engineering, Nanyang Technological University

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A protocol for small molecular drug screening based on in-situ synthesis of ultrasmall fluorescent gold nanoclusters (Au NCs) using drug-loaded protein as template is presented. This method is simple to determine the binding affinity of drugs to a target protein by a visible fluorescent signal emitted from the protein-templated Au NCs.

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Fluorimetric Method Protein-targeting Small Molecule Drug Screening Binding Affinity Drug Molecules Fluorescent Gold Nanoclusters (Au NCs) Albumin Proteins Human Serum Albumin (HSA) Bovine Serum Albumin (BSA) Small Molecular Drugs Ibuprofen Warfarin Phenytoin Sulfanilamide Formation Rate Of Fluorescent Au NCs Denaturing Conditions Urea Fluorescent Intensity Drug-protein Binding Affinity Rate Of Au NCs Formation Fluorescence Intensity Measurement Relative Binding Strength Drug-protein Binding Constant (KD) Drug Content Preloaded
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