Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Pei-Ju Hsu; Ko-Jiunn Liu; Ying-Yin Chao; Huey-Kang Sytwu; B. Linju Yen

doi:10.3791/53265

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

人骨髓间充质干细胞（干细胞）的免疫调节性能评估

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06:20 min

December 24, 2015

DOI: 10.3791/53265-v

Pei-Ju Hsu¹, Ko-Jiunn Liu², Ying-Yin Chao¹, Huey-Kang Sytwu³, B. Linju Yen¹

¹Regenerative Medicine Research Group, Institute of Cellular & System Medicine,National Health Research Institutes (NHRI), ²National Institute of Cancer Research,National Health Research Institutes (NHRI), ³Institute of Microbiology & Immunology,National Defense Medical Center

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人类骨髓间充质干细胞（MSC）的免疫调节属性显示越来越重要的临床应用。用MSC和外周血白细胞的共同培养系统的预染色的荧光染料羧基琥珀酰亚胺酯（CFSE），我们描述对效应白细胞增殖和特定亚群的体外评估的MSC免疫调节的。

本实验的总体目标是评估人间充质干细胞或 MSC 的免疫调节特性。这种方法可以帮助阐明人间充质干细胞免疫调节所涉及的机制，这一过程可能具有治疗相关性。该技术的主要优点是可以评估效应子的发生、白细胞增殖和细胞分裂的数量，证明该程序将是每只鞋。

我实验室的技术人员要分离 PBMC，首先在 50 毫升锥形管中用 25 毫升 PBS 稀释 25 毫升肝素化全血样品。接下来，向新的 50 mL 试管中加入 15 mL phi，称为堆积密度梯度。然后将试管保持一定角度，将稀释的细胞悬液缓慢地置于密度梯度上。

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免疫学第106 间质干细胞（MSC）人免疫调节白细胞增殖羧基琥珀酰亚胺酯（CFSE）流式细胞术