Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

Eva Porlan; Beatriz Mart&#237;-Prado; Antonella Consiglio; Isabel Fari&#241;as

doi:10.3791/53282

在成年小鼠V-SVZ细胞的神经分析的稳定，高效的基因修饰干细胞自主和非自主的影响

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Developmental Biology

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JoVE Journal Developmental Biology

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

在成年小鼠V-SVZ细胞的神经分析的稳定，高效的基因修饰干细胞自主和非自主的影响

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08:48 min

February 17, 2016

DOI: 10.3791/53282-v

Eva Porlan¹, Beatriz Martí-Prado^2,3, Antonella Consiglio^4,5, Isabel Fariñas^2,3

¹Cell Division and Cancer Group,Spanish National Cancer Research Centre (CNIO), ²Centro de Investigaciones Biomédicas en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ³Departmento de Biologìa Celular,Universidad de Valencia, ⁴Institut de Biomedicina de la Universitat de Barcelona (IBUB), ⁵Department of Molecular and Translational Medicine, Fibroblast Reprogramming Unit,University of Brescia

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在这里，我们描述了一种基于使用慢病毒颗粒对成体脑室-脑室下神经源性生态位中的神经干细胞和/或其相邻室管膜细胞进行长期基因修饰的程序，该程序允许单独分析细胞自主和非自主、生态位依赖性对神经干细胞的影响。

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发育生物学第108 神经再生心室脑室下区室管膜下区侧脑室神经干细胞室管膜细胞利基慢病毒。