Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

Charles J. Benck; Tijana Martinov; Brian T. Fife; Devavani Chatterjea

doi:10.3791/53638

Journal

/

免疫与感染

/

从小鼠皮肤使用酶消化和梯度分离提取白细胞浸润的

Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

JoVE 杂志
免疫与感染

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志免疫与感染

Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

从小鼠皮肤使用酶消化和梯度分离提取白细胞浸润的

Please note that all translations are automatically generated. Click here for the English version.

21,203 Views

•

07:11 min

•

January 25, 2016

DOI:

10.3791/53638-v

DOI:

10.3791/53638-v

•

07:11 min

January 25, 2016

•

7 Views

Charles J. Benck¹, Tijana Martinov², Brian T. Fife², Devavani Chatterjea¹

¹Department of Biology,Macalester College, ²Department of Medicine, Division of Rheumatic and Autoimmune Diseases, Center for Immunology,University of Minnesota

成績單

Summary

This protocol describes enzymatic digestion of mouse skin in nutrient-rich medium followed by gradient separation to isolate leukocytes. Cells thus derived can be used for diverse downstream applications. This is an effective, economical, and improved alternative to tissue dissociation machines and harsher trypsin and dispase-based tissue digestion protocols.