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提高2D和3D皮肤<em>体外</em>模型使用大分子拥挤

Improving 2D and 3D Skin <em>In Vitro</em> Models Using Macromolecular Crowding

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Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

提高2D和3D皮肤<em>体外</em>模型使用大分子拥挤

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12,436 Views

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09:14 min

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August 22, 2016

DOI:

10.3791/53642-v

DOI:

10.3791/53642-v

•

09:14 min

August 22, 2016

•

12424 Views

Paula Benny^1,2, Cedric Badowski², E. Birgitte Lane², Michael Raghunath^1,2,3

¹Department of Biochemistry, Yong Loo Lin School of Medicine,National University of Singapore ²Epithelial Biology Laboratory,Institute of Medical Biology, A*STAR ³Department of Biomedical Engineering, Faculty of Engineering,National University of Singapore

Summary

我们提出了一个协议，以获得富含胞外基质蛋白的细胞来源的基质，使用大分子沟渠扫污机（MMC）。此外，我们提出该结合MMC在三维器官皮肤共培养产生，从而降低了培养时间，同时保持结构的成熟的协议。

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