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Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Article DOI: 10.3791/53749-v • 09:09 min • December 17th, 2015
December 17th, 2015 •
1, 3, 2, 4, 1
1Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College London, 2Academic Department of Rheumatology, Centre for Molecular and Cellular Biology of Inflammation, Division of Immunology, Infection and Inflammatory Disease, King's College London, 3ARC Centre for Advanced Molecular Imaging, Australian Centre for NanoMedicine, University of New South Wales Australia, 4Departments of Chemistry and Physic, McGill University

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To investigate flow velocities and directionality of filamentous-actin at the T cell immunological synapse, live-cell super-resolution imaging is combined with total internal reflection fluorescence and quantified with spatio-temporal image correlation spectroscopy.

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Cortical Actin Flow T Cells Spatio-temporal Image Correlation Spectroscopy Structured Illumination Microscopy Data Filamentous-actin Motility Immune Cells Immunological Synapse F-actin Molecular Distributions Sub-membranous Vesicle Dynamics Protein Clustering Light Microscope Techniques Super Resolution Imaging Total Internal Reflection Fluorescence Two-dimensional Molecular Flow Velocity Histograms Vector Maps
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