Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

George Ashdown; Elvis Pand&#382;i&#263;; Andrew Cope; Paul Wiseman; Dylan Owen

doi:10.3791/53749

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

皮层肌动蛋白流通过结构照明显微镜数据的时空图像相关光谱量化性T细胞

Full Text

10,207 Views

09:09 min

December 17, 2015

DOI: 10.3791/53749-v

George Ashdown¹, Elvis Pandžić³, Andrew Cope², Paul Wiseman⁴, Dylan Owen¹

¹Department of Physics and Randall Division of Cell and Molecular Biophysics,King's College London, ²Academic Department of Rheumatology, Centre for Molecular and Cellular Biology of Inflammation, Division of Immunology, Infection and Inflammatory Disease,King's College London, ³ARC Centre for Advanced Molecular Imaging, Australian Centre for NanoMedicine,University of New South Wales Australia, ⁴Departments of Chemistry and Physic,McGill University

Please note that some of the translations on this page are AI generated. Click here for the English version.

为了研究丝状肌动蛋白在 T 细胞免疫突触处的流速和方向性，将活细胞超分辨率成像与全内反射荧光相结合，并用时空图像相关光谱进行量化。

本实验的总体目标是在通过全内反射、结构照明成像或草坪模拟采集后，通过图像相关光谱量化皮质肌动蛋白的动力学。这种方法可以帮助回答生物物理领域的关键问题，例如皮质肌动蛋白流在调节免疫突触的相互作用中的作用。该技术的主要优点是它将纳米级分辨率与生活方式兼容性相结合，与传统的实例显微镜技术相比，可以观察和分析更小尺度的过程。

演示这项技术的将是我实验室的博士生 George Ashdown。对于测试样品，将 5 微升直径为 100 纳米的亚衍射荧光微球储备液添加到新鲜未涂层的盖玻片的表面。然后用移液管吸头将微球溶液涂抹在盖玻片上并使其干燥。

View the full transcript and gain access to thousands of scientific videos