An Improved Method for Rapid Intubation of the Trachea in Mice

Tyler C. Vandivort; Dowon An; William C. Parks

doi:10.3791/53771

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An Improved Method for Rapid Intubation of the Trachea in Mice

在小鼠气管插管快速的改进方法

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08:46 min

February 22, 2016

DOI: 10.3791/53771-v

Tyler C. Vandivort^1,2, Dowon An³, William C. Parks²

¹Department of Environmental and Occupational Health Sciences,University of Washington, ²Department of Medicine,Cedars-Sinai Medical Center, ³Division of Pulmonary and Critical Care Medicine,University of Washington

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本文介绍了通过插管直接施用博莱到小鼠气管一种快速，简便的方法。这种方法的主要优点是，它是高度可再现的，容易掌握，并且不需要专门的设备或冗长的恢复时间。

该程序的总体目标是通过插管将试剂快速、简单和可靠地注入小鼠肺。该方法还限制了对周围组织造成残留损伤的风险，并且价格低廉。这种方法可以帮助回答基础肺生物学和疾病领域机制中的关键问题。

如急性肺损伤和纤维化。该技术的主要优点是高度可复制、易于掌握、不需要专门的设备或漫长的恢复时间。该程序必须使用消毒工具进行。

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医药第108 鼠标肺纤维化博莱霉素气管内插管非手术非侵入性异氟烷