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Aislamiento de CD146
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Aislamiento de CD146

Article DOI: 10.3791/53782-v • 09:47 min • June 17th, 2016
June 17th, 2016 •
1,2, 1,3,4, 1,2,5
1Sinclair Centre for Regenerative Medicine, Ottawa Hospital Research Institute, 2University of Ottawa, 3Department of Neonatology and Pediatric Critical Care Medicine, Medical Faculty and University Hospital Carl Gustav Carus, Technische Universität Dresden, 4DFG Research Center and Cluster of Excellence for Regenerative Therapies (CRTD), Technische Universität, Dresden, 5Children's Hospital of Eastern Ontario Research Institute

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Este protocolo describe una técnica de aislamiento para obtener células mesenquimales del estroma residentes de pulmón primarios de ratas, mediante el uso de la digestión enzimática, la separación por gradiente de densidad, la adherencia de plástico y CD146 + selección de perlas magnéticas.

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Biología Celular Número 108 de vástago mesenquimales células del estroma células de tejido residentes madre / progenitoras de pulmón de la rata la medicina regenerativa la selección de perlas magnéticas
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