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Chapters
- 00:05Title
- 00:46Preparation of Agrobacterium Infiltration Solution
- 01:48Tobacco Plant and Leaf Injection
- 03:12Confocal Imaging
- 04:45Image Processing and Quantification Analysis
- 06:53Results: Spatial Reorganization of MAPK Signaling Pathway in Tobacco Cells
- 07:42Conclusion
At the subcellular level, signaling events are dynamically modulated by developmental and environmental cues. Here we describe a protocol that employs the tobacco transient expression system to monitor dynamic protein-protein interaction and to disclose spatial organization of signal transduction in plant cells.
