Isolation and Characterization of Single Cells from Zebrafish Embryos

Leigh Ann Samsa; Nicole Fleming; Scott Magness; Li Qian; Jiandong Liu

doi:10.3791/53877

Journal

/

发育生物学

/

分离和斑马鱼胚胎单细胞的表征

Isolation and Characterization of Single Cells from Zebrafish Embryos

JoVE 杂志
发育生物学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志发育生物学

Isolation and Characterization of Single Cells from Zebrafish Embryos

分离和斑马鱼胚胎单细胞的表征

Please note that all translations are automatically generated. Click here for the English version.

14,518 Views

•

09:25 min

•

March 12, 2016

DOI:

10.3791/53877-v

DOI:

10.3791/53877-v

•

09:25 min

March 12, 2016

•

14494 Views

Leigh Ann Samsa^1,2, Nicole Fleming^2,3, Scott Magness¹, Li Qian^2,3, Jiandong Liu^2,3

¹Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill ²McAllister Heart Institute,University of North Carolina at Chapel Hill ³Department of Pathology and Laboratory Medicine,University of North Carolina at Chapel Hill

Summary

This protocol describes a method for isolating single cells from zebrafish embryos, enriching for cells of interest, capturing zebrafish cells in microfluidic based single cell multiplex systems, and assessing gene expression from single cells.

Explore More Videos

Single Cell Isolation Zebrafish Embryos Microfluidic-based Single-cell Analysis Gene Expression 发育生物学 Cell Heterogeneity Cell Dissociation Cell Preparation Chorion Removal De-yolking Cell Washing Cell Resuspension

分离和斑马鱼胚胎单细胞的表征

Summary

Automatically generated

Explore More Videos

Related Videos