Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Despina Soteriou; Lana Kostic; Egor Sedov; Yahav Yosefzon; Hermann Steller; Yaron Fuchs

doi:10.3791/53931

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

从背小鼠皮肤分离毛囊干细胞和表皮角质形成细胞

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06:51 min

April 29, 2016

DOI: 10.3791/53931-v

Despina Soteriou¹, Lana Kostic¹, Egor Sedov¹, Yahav Yosefzon¹, Hermann Steller², Yaron Fuchs¹

¹Department of Biology and Lokey Center, Laboratory of Stem Cell Biology and Regenerative Medicine,Technion Israel Institute of Technology, ²Strang Laboratory of Apoptosis and Cancer Biology,Howard Hughes Medical Institute, The Rockefeller University

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研究成体干细胞生物学的理想模型是小鼠毛囊。在这里，我们提出了一种分离不同毛囊干细胞和表皮角质形成细胞群的方案，采用小鼠背皮的酶消化，然后进行 FACS 分析。

该方案的总体目标是从小鼠背侧皮肤中分离出不同的干细胞和表皮角质形成细胞群。该协议允许用户同时从小鼠背侧皮肤中分离多个细胞群。该技术的主要优点是快速、可靠，可用于从异质性皮肤细胞群的混合物中分离特定的目标细胞类型。

演示该程序的将是我实验室的 Yahav Yosefzon 博士。首先使用电动剪刀剃掉处于毛囊周期休止期的 50 至 80 日龄小鼠的所有背侧皮肤毛发，使剪刀尽可能靠近皮肤，而不会损坏皮肤和皮下层。接下来，使用 70% 乙醇对皮肤进行消毒并去除任何头发残留物。

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发育生物学第110 干细胞毛囊鼠标背部皮肤切开细胞分选流式细胞仪