Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of <em>Xenopus laevis</em>

Alexander Brinkmann; Camille Okom; Eugen Kludt; Detlev Schild

doi:10.3791/54108

Journal

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神经科学

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通过的嗅球钙监控录像的变化温度引起的神经元电活动<em>非洲爪蟾</em

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of <em>Xenopus laevis</em>

JoVE 杂志
神经科学

This content is Free Access.

JoVE 杂志神经科学

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis

通过的嗅球钙监控录像的变化温度引起的神经元电活动<em>非洲爪蟾</em

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7,258 Views

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11:08 min

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June 03, 2016

DOI:

10.3791/54108-v

DOI:

10.3791/54108-v

•

11:08 min

June 03, 2016

•

7246 Views

Alexander Brinkmann*^1,2, Camille Okom*^1,2, Eugen Kludt^1,2,4, Detlev Schild^1,2,3

¹Institute of Neurophysiology and Cellular Biophysics,Georg-August-Universität Göttingen ²Center for Nanoscale Microscopy and Molecular Physiology of the Brain,Georg-August-Universität Göttingen ³DFG Excellence Cluster 171,Georg-August-Universität Göttingen ⁴German Hearing Center Hannover

Summary

Here we describe a protocol for measuring and analyzing temperature responses in the olfactory bulb of Xenopus laevis. Olfactory receptor neurons and mitral cells are differentially stained, after which calcium changes are recorded, reflecting a sensitivity of some neural networks in the bulb to temperature drops induced at the nose.