Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Haseong Kim; Gui Hwan Han; Yaoyao Fu; Jongsik Gam; Seung Goo Lee

doi:10.3791/54144

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

高度敏感和快速荧光检测用便携式分析仪FRET

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08:27 min

October 1, 2016

DOI: 10.3791/54144-v

Haseong Kim*¹, Gui Hwan Han*¹, Yaoyao Fu¹, Jongsik Gam², Seung Goo Lee^1,3

¹Synthetic Biology & Bioengineering Research Center,Korea Research Institute of Bioscience and Biotechnology, ²College of Interdisciplinary & Creative Studies,Konyang University, ³Biosystems and Bioengineering Program,University of Science and Technology

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这个协议描述了使用定制便携式FRET分析器荧光共振能量转移（FRET）的传感器数据的快速且高灵敏度定量。该装置被用于最大化的检测灵敏度，有利的糖含量的实际和有效的评估的临界温度范围内检测麦芽糖。

该方案的总体目标是通过量化市售饮料的糖含量来证明 FRET 分析仪在最佳条件下的普遍适用性。这种方法可以帮助回答小分子（如糖）传感和监测领域的关键问题。该技术的主要优点是它可以有效地监测响应小分子的新信号，而无需昂贵的高端荧光计。

演示该程序的将是来自我们实验室的 Han 博士。首先，制备麦芽糖或蔗糖特异性青色和黄色荧光生物传感器质粒。然后用单个 DE3 大肠杆菌菌落接种 5 毫升 Luria 肉汤，并在 37 摄氏度下在摇动培养箱中孵育培养物 16 小时。

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生物化学第116 荧光共振能量转移便携式装置床边血糖检测糖度荧光计食品评估荧光共振能量转移（FRET）的