用于木质纤维素生物转化乙醇的耐受性戊糖发酵酵母的进化技术
11.2K 次观看
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被引用 4 次
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14:53 分钟
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2016年10月24日
DOI :10.3791/54227-v
2016年10月24日
•11.2K 次观看
描述并演示了适应性进化和分离技术，以获得能够快速消耗酶解未经脱毒的水解物中混合的己糖和戊糖，并积累超过40 g/L乙醇的Scheffersomyces stipitis菌株NRRL Y-7124的衍生菌株。
Chapters in this video
0:05
Title
1:40
Enrichment of Robust Derivatives during Serial Transfer on AFEX CSH
3:42
Isolation of Single Cell Tolerant Derivatives after Enrichment on AFEX CSH
4:58
Performance Evaluation of AFEX CSH Tolerant Derivatives Compared to Parent
7:15
Applying Continuous Culture to Select for Ethanol-challenged Xylose Utilization
9:43
Isolation of Single-cell Colonies That Utilize Xylose in PSGHL When Ethanol Is Present
11:20
Results: Derivatives of S. stipitis Parent Strain Have Improved Efficiency in Converting Hydrolyzates to Ethanol
12:54
Conclusion
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