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JoVE Journal
Immunology and Infection
人体血液中测粒及单核细胞吞噬功能和呼吸爆发活动
人体血液中测粒及单核细胞吞噬功能和呼吸爆发活动
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood

人体血液中测粒及单核细胞吞噬功能和呼吸爆发活动

Full Text
14,622 Views
11:29 min
September 12, 2016

DOI: 10.3791/54264-v

Mary Pat Meaney1, David C. Nieman1, Dru A. Henson2, Qi Jiang3, Fu-Zhang Wang3

1Human Performance Laboratory, North Carolina Research Campus,Appalachian State University, 2Department of Biology,Appalachian State University, 3David H. Murdock Research Institute

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Please note that some of the translations on this page are AI generated. Click here for the English version.

本手稿的目的是提出一种测量人体血液样本中单核细胞和粒细胞吞噬作用以及氧化爆发活性的方法。

该测定的总体目标是测量全血中的粒细胞和单核细胞吞噬作用以及氧化爆发活性。这种方法可以帮助回答免疫学领域的关键问题,例如特定的饮食干预如何影响对运动的免疫反应。该技术的主要优点是它是一种简单、直接的检测方法,可以很容易地进行修改,以适应可用的实验室资源和研究兴趣。

在过去的二十年里,我们一直在许多运动试验中使用这种先天免疫功能的测量方法,我们将在视频后面分享其中的一些数据。在开始程序之前,根据制造商的说明,使用血液分析仪对血液样本进行全血细胞计数和白细胞鉴别分析。记下每毫升细胞数的白细胞计数以及样品的中性粒细胞和单核细胞百分比值。

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