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ट्रोफोब्लास्ट स्टेम कोशिकाओं में Murine भ्रूण fibroblasts की प्रत्यक्ष रूपांतरण के लिए प्रोटोकॉल
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
DOI:

08:57 min

July 25, 2016

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Chapters

  • 00:05Title
  • 00:40293T Cell Lentiviral Vector Production
  • 03:06Fibroblast to Induced Trophoblast Stem Cell (iTSC) Conversion
  • 05:10Individual iTSC Line Isolation (Transdifferentiation Day 21-28)
  • 07:02Results: Representative Morphological Changes During MEF to iTSC Conversion
  • 07:56Conclusion

Summary

自动翻译

Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.

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