Fluorescent Orthotopic Mouse Model of Pancreatic Cancer

Jonathan A. Moreno; Antonio Sanchez; Robert M. Hoffman; Saima Nur; Maria P. Lambros

doi:10.3791/54337

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Fluorescent Orthotopic Mouse Model of Pancreatic Cancer

胰腺癌的荧光原位小鼠模型

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06:48 min

September 20, 2016

DOI: 10.3791/54337-v

Jonathan A. Moreno¹, Antonio Sanchez², Robert M. Hoffman^3,4, Saima Nur¹, Maria P. Lambros¹

¹College of Pharmacy,Western University of Health Sciences, ²UVP, LLC, ³Department of Surgery, School of Medicine,University of California San Diego, ⁴Anticancer Inc.

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一种将表达绿色荧光蛋白的胰腺癌细胞（PANC-1 GFP）原位植入 Balb-c Ola Hsd-Fox1nu 小鼠胰腺以评估肿瘤进展和转移的程序。

该程序的总体目标是开发荧光胰腺癌的小鼠原位模型，该模型可以在活体动物中进行无创监测。该模型允许对感兴趣的特定药物对原发性肿瘤及其在活体动物中的转移的影响进行非侵入性研究。这种技术的主要优点是可以在原位很容易观察到处理的效果。

首先根据制造商的说明解冻等分试样的高浓度 Matrigel。接下来，用 5 毫升 DPBS 洗涤每个细胞瓶，并用胰蛋白酶收获培养物。加入 RPMI 培养基后，将所得细胞悬液转移到 15 mL 试管中，然后离心细胞。

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癌症研究第115 肿瘤生物学发行原位模型裸鼠胰腺癌在体内荧光成像癌症动物模型细胞培养行剖腹探查术术