Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Edward O. Kwarteng; Krista M. Heinonen

doi:10.3791/54345

Journal

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发育生物学

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竞争性移植评价造血干细胞健身

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

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发育生物学

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JoVE 杂志发育生物学

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

竞争性移植评价造血干细胞健身

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15,154 Views

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08:53 min

•

August 31, 2016

DOI:

10.3791/54345-v

DOI:

10.3791/54345-v

•

08:53 min

August 31, 2016

•

21 Views

Edward O. Kwarteng¹, Krista M. Heinonen¹

¹INRS-Institut Armand-Frappier, Université du Québec,Institut National de la Recherche Scientifique

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Summary

This protocol provides step-by-step guidelines for setting up competitive mouse bone marrow transplant experiments to study hematopoietic stem/progenitor cell function without prior purification of stem cells by cell sorting.

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Competitive Transplant Assay Hematopoietic Stem Cells Bone Marrow Stem Cell Fitness Self-renewal Mixed Bone Marrow Chimeras Immunology Cell Suspension Biological Safety Cabinet Surgical Platform Sterile PBS Cell Counting Centrifugation Donor Equivalents

竞争性移植评价造血干细胞健身

成績單

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Summary

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